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Valery Khramtsov, PhD

Staff photo

473 W 12th Ave
Columbus, OH 43210

Phone: (614) 688-3664

Email: khramtsov.1@osu.edu


Current OSU Appointment

Associate Professor, Pulm,Allergy,Crit Care & Sleep



Dr. Khramtsov is an Associate Professor in the Department of Internal Medicine, The Ohio State University. He received his M.S. from the Novosibirsk State University and his Ph.D. from the Institute of Chemical Kinetics & Combustion, Novosibirsk, Russia. He received the State Award of Russian Federation in Science and Technology, 1994, honored with the gold medal presenting by the president of the country. He was Fellow of Alexander von Humboldt foundation at the Max-Planck Institute for Biophysical Chemistry, Götiingen, Germany (1990-1991), visiting Professor at Johann Wolfgang Goethe University, Frankfurt, Germany (1996) and Fellow of Fogarty International Center at the Ohio State University, Columbus, OH (1997-1998). Since 2001, Dr. Khramtsov is Investigator at Dorothy M. Davis Heart & Lung Research Institute, Columbus, OH, and since 2009 he is Associate Professor at the Division of Pulmonary, Allergy, Critical Care and Sleep Medicine. He has authored more than 100 peer-reviewed articles and book chapters dealing with free radical biomedicine and magnetic resonance applications to chemistry and biology. His research was supported by numerous international (NATO, INTAS, CRDF) and national (NIH) grants. Dr. Khramtsov served at several NIH study sections in 2010 and 2011. He is a member of Editorial Boards of journals "Advances in Molecular Imaging" and "Applied Magnetic Resonance". Dr. Khramtsov teaches Free Radical in Biomedicine course at the Internal Medicine and Biophysical programs of the OSU. He mentored and trained more than 20 fellows and graduate students, most of them defended Ph.D. thesis under his supervision and successfully continue their academic career in leading research institutes in the USA, France and Russia.

Chapters in Books

Khramtsov, VV, Weiner, LM.. ". Proton exchange in stable nitroxyl radicals: pH-sensitive spin probes." In Imidazoline Nitroxides. Vol. 2. Edited by Volodarsky, L.B.. 37-80. Boca Raton, FL, US|USA: CRC Press, June 1988.

Dikalov, S, Khramtsov, VV, Zimmer G.. "Reaction of lipoate and its derivatives with reactive oxygen species." In Lipoic Acid in Health and Disease. Edited by Fuchs, J, Packer, L.. 47-66. New York, NY, US|USA: Marcel Dekker, January 1997.

Khramtsov, VV, Volodarsky, LB.. "Use of imidazoline nitroxides in studies of chemical reactions: ESR measurements of concentration and reactivity of protons, thiols and nitric oxide." In Spin Labeling: The Next Millennium. Edited by Berliner, LJ. 109-180. New York, NY, US|USA: Plenum Press, June 1998.

Khramtsov, VV. "New Approaches in Spin Labeling and Spin Trapping Part One: ESR Studies of Local Chemical Environment." In Supramolecular Structure and Function 7. Edited by Pifat, G.. 107-118. New York, Boston, Dordrecht, London, Moscow: Kluwer/Academic/Plenum Publishers, May 2001.

Khramtsov VV, Berliner LJ, Clanton TL.. "New Approaches in Spin Labeling and Spin Trapping Part Two: NMR Detects Free Radicals." In Supramolecular Structure and Function 7. Edited by Pifat, G.. 107-118. New York, Boston, Dordrecht, London, Moscow: Kluwer/Academic/Plenum Publishers, May 2001.

Berliner LJ, Khramtsov V, Fuji H, Clanton TL.. "Unique Applications of Spin Traps As Indicators of Oxidative Stress in Living Systems." In Bio-Assays for Oxidative Stress Status. Edited by Pryor, W.A.. 262-272. Amsterdam: Elsevier, November 2001.

Lurie DJ, Foster MA, Youngdee W, Khramtsov V V, Grigor'ev IA.. "Recent progress and future prospects of free radical imaging by PEDRI." In EPR in the 21st Century. Edited by Kawamori A, Yamauchi J, Ohta H.. 515-523. Amsterdam: Elsevier, June 2002.

Khramtsov, VV. "Functional EPR spectroscopy and imaging of nitroxides." In Supramolecular Structure and Function 9. Edited by Pifat-Mrzljak, G.. 181-208. New York, Boston, Dordrecht, London, Moscow: Kluwer/Academic/Plenum, June 2007.

Khan N., Khramtsov, V.V. and Swartz, H.M.. "Methods for Tissue pO2, Redox, pH, and Glutathione Measurements by EPR Spectroscopy." In Methods in Redox Signaling. Edited by Das, D.. 79-87. New Rochelle, NY, US|USA: Mary Ann Liebert, Inc., June 2009.

Khramtsov, VV, Zweier, JL. "Functional in vivo EPR Spectroscopy and Imaging Using Nitroxide and Trityl Radicals." In Stable Radicals: Fundamentals and Applied Aspects of Odd-Electron Compounds. Edited by Hicks, R.. 537-566. New York, NY, US|USA: John Wiley & Sons, April 2010.

Khramtsov, V.V. and Komarov, D.A.. "Nanospin Probes and Applications to Cardiology." In Nanomedicine and the Cardiovascular System. Edited by Hunter RJ, Preedy, VR. 200-220. Enfield, NH, US|USA: Science Publisher and CRC Press, July 2011.

Khramtsov VV. "In vivo Spectroscopy and Imaging of Nitroxide Probes." In Nitroxides - Theory, Experiment and Applications. Edited by Kokorin, A.I.. 317-346. Rijeka, HR|HRV: InTech, September 2012.



1980 M.S., Novosibirsk State University

1986 Ph.D., Novosibirsk State University


Editorial Activities

2006 - present Applied Magnetic Resonance
2011 - present Advances in Molecular Imaging


1987 All-Union Mendeleev Chemical Society Award.
1989 Fellowship. Alexander von Humboldt Foundation, Germany.
1994 State Award of Russian Federation (gold medal).
1997 Research Fellowship. Fogarty International Center, USA.

Journal Articles

Gruzdev, AG, Khramtsov V V, Weiner LM, Budker, VG. "Fluorescence polarization study of the interaction of biopolimers with liposomes." FEBS Letters. Vol. 137, (January 1982.): 227-230.

Khramtsov VV, Weiner, LM, Grigor'ev, IA, Volodarsky, LB. "Proton exchange in stable nitroxyl radicals. EPR study of the pH of aqueous solutions.." Chem. Phys. Letters. Vol. 91, (January 1982.): 69-72.

Grigor'ev LA, Schukin GI, Khramtsov V V, Weiner LM, Starichenko VF, Volodarsky LB. "Transformation of nitroxyl radicals of 3-inidazoline-3-oxide in nitronylnitroxyl radicals." Izv, AN.SSSR, ser. khimicheskaja (Russia). (January 1985.): 2342-2351.

Khramtsov V V, Weiner LM, Grigor'ev LA, Reznikov VA, Schukin GI, Volodarsky LB. "The influence of protonation and deprotonation of functional groupd of nitroxyl radicals on their EPR spectra." Khimicheskaja Fizika (Russia). (January 1985.): 637-643.

Khramtsov V V, Weiner LM, Eremenko SI, Belchenko OI, Schastnev PV, Grigor'ev LA, Reznikov VA. "Proton exchange in stable nitroxyl radicals of imidazoline and imidazolidine series." J. Magn. Res.. Vol. 61, (January 1985.): 397-408.

Khramtsov V V, Weiner LM, Gogolev AZ, Grigor'ev IA, Starichenko VK, Volodarsky LB. "ESR and PMR studies of a new class of nitroxyl, nitronylnitroxyl and iminonitroxyl radicals." Magn. Reson. Chem.. Vol. 24, (January 1986.): 199-207.

Khramtsov V V, Weiner LM. "Kinetic parameters of proton exchange in stable nitroxyl radicals." Khimicheskaja Fizika (Russia). Vol. 6, (January 1987.): 499-505.

Khramtsov, V. V. and Weiner, L.M.. "Proton transfer reactions in free Radicals. Spin pH Probes." Russian Chemical Reviews. Vol. 57, no. 9. (January 1988.): 824-839.

Khramtsov, V, V; Yelinova, V, I; Weiner, L, M; Berezina, T, A; Martin, V, V; Volodarsky, L B. "Quantitative determination of SH groups in low- and high-molecular-weight compounds by an electron spin resonance method.." Analytical biochemistry. Vol. 182, no. 1. (January 1989.): 58-63.

Khramtsov, V, V; Panteleev, M, V; Weiner, L M. "ESR study of proton transport across phospholipid vesicle membranes.." Journal of biochemical and biophysical methods. Vol. 18, no. 3. (January 1989.): 237-46.

Khramtsov, V, V; Vainer, L, M; Rar, V, A; Berezina, T, A; Martin, V, V; Volodarskii, L B. "[Macromolecular spin pH-probes on the basis of human serum albumin]." Biokhimii?a? (Moscow, Russia). Vol. 55, no. 6. (January 1990.): 1014-7.

Berezina TA, Martin VV, Volodarsky LB, Khramtsov VV, Weiner LM. "Synthesis of amidine derivatives of imidazoline nitroxyl radicals-new series of pH-sensitive spin probes and labels." Bioorganicheskaja Khimija (Russia). Vol. 16, (January 1990.): 262-269.

Khramtsov, V, V; Marsh, D. "Measurement of the local translational diffusion rates of proteins by saturation transfer EPR spectroscopy.." Biochimica et biophysica acta. Vol. 1068, no. 2. (January 1991.): 257-60.

Khramtsov, V, V; Gorunova, T, E; Weiner, L M. "Monitoring of enzymatic activity in situ by EPR.." Biochemical and biophysical research communications. Vol. 179, no. 1. (January 1991.): 520-7.

Khramtsov VV, Yelinova VI, Goryunova TE, Weiner LM. "Quantitative determinationand Reversible Modification of Sulfhydryl Groups in Low-and High-Molecular-Weight Compounds using Biradical Spin Label." Biochemistry (Moscow). Vol. 56, (January 1991.): 1567-1577.

Khramtsov, V, V; Marsh, D; Weiner, L; Reznikov, V A. "The application of pH-sensitive spin labels to studies of surface potential and polarity of phospholipid membranes and proteins.." Biochimica et biophysica acta. Vol. 1104, no. 2. (January 1992.): 317-24.

Balakirev M Yu, Khramtsov V V, Berezina TA, Martin VV, Volodarsky LB. "The synthesis of amidine derivatives of imidazoline nitroxides-a series of pH-sensitive spin probes and labels." Synthesis. Vol. 12, (January 1992.): 1223-1225.

Balakirev M Yu, Khramtsov V V. "New pH-sensitive aminoxyls-application to the study of biomembrane transport processes." J. Chem. Sos. Perkin Trans. 2. Vol. 11, (January 1993.): 2157-2160.

Khramtsov, V, V; Grigor'ev, I, A; Kiriljuk, I, A; Utepbergenov, D I. "Spin trapping of nitric oxide by nitronylnitroxides: measurement of the activity of no synthase from rat cerebellum.." Biochemical and biophysical research communications. Vol. 202, no. 1. (January 1994.): 195-203.

Khramtsov V V, Woldman YaYu, Grigo'ev IA, Kiriljuk IA, Utepbergenov DI. "Trapping of nitric oxide and nitric oxide synthase activity measurement by nitronylnitroxyl radicals." Magnetic Reson. in Medicine. Vol. 6, (January 1995.): 282-284.

Utepbergenov, D, I; Khramtsov, V, V; Vlassenko, L, P; Markel, A, L; Mazhukin, D, G; Mazhukin, D, G; Volodarsky, L B. "Kinetics of nitric oxide liberation by 3,4-dihydro-1,2-diazete 1,2-dioxides and their vasodilatory properties in vitro and in vivo.." Biochemical and biophysical research communications. Vol. 214, no. 3. (January 1995.): 1023-32.

Zamaraev KI, Salganik RI, Romannikov VN, Wlassoff WA, Khramtsov V V. "Modelling of Prebiotic Oligopeptide synthesis in the Presence of Zeolites and Kaolin as Catalysis." Kokladi Akademii Nauk (Russia). Vol. 340(6), (January 1995.): 779-782.

Yelinova VI, Khramtsov V V, Salganik RI, Solovjova NA, Kudrjashova LN. "EPR measurement of thiols in the human and rat blood as a method for diagnosis of oxidative damage processes." Magnetic Reson. in Medicine. Vol. 6, (January 1995.): 285-287.

Slepneva, I, A; Sergeeva, S, V; Khramtsov V V. "Reversible inhibition of NADPH-cytochrome P450 reductase by alpha-lipoic acid.." Biochemical and biophysical research communications. Vol. 214, no. 3. (January 1995.): 1246-53.

Sergeeva SV, Slepneva IA, Khramtsov V V, Zimmer G, Ulrich H. "Inhibition of enzymes of microsomal monoxigenase system by a-Lipoic and selenolipoic acids." Exp. Toxic Pathol.. Vol. 48, (January 1996.): 361-365.

Paschenko, S, V; Khramtsov, V, V; Skatchkov, M, P; Plyusnin, V, F; Bassenge, E. "EPR and laser flash photolysis studies of the reaction of nitric oxide with water soluble NO trap Fe(II)-proline-dithiocarbamate complex.." Biochemical and biophysical research communications. Vol. 225, no. 2. (January 1996.): 577-84.

Dikalov, S; Khramtsov, V; Zimmer, G. "Determination of rate constants of the reactions of thiols with superoxide radical by electron paramagnetic resonance: critical remarks on spectrophotometric approaches.." Archives of biochemistry and biophysics. Vol. 326, no. 2. (January 1996.): 207-18.

Yelinova, V; Glazachev, Y; Khramtsov, V; Kudryashova, L; Rykova, V; Salganik, R. "Studies of human and rat blood under oxidative stress: changes in plasma thiol level, antioxidant enzyme activity, protein carbonyl content, and fluidity of erythrocyte membrane.." Biochemical and biophysical research communications. Vol. 221, no. 2. (January 1996.): 300-3.

Balakirev M Yu, Khramtsov V V. "EPR study of free radical decomposition of NN-bis(arilsulfonil) hydroxylamines inorganic solution." J. Organic Chemistry. Vol. 61, (January 1996.): 7263-7269.

Khramtsov, V, V; Utepbergenov, D, I; Vol'dman, Ia Iu; Vlasenko, L, P; Markel', A, L; Kiriliuk, I, A; Grigor'ev, I, A; Mazhukin, D, G; Tikhonov, A Ia; Volodarskii, D G. "[Studies of in vitro and in vivo derived 1,2-diazetine and nitronylnitroxide as donors and acceptors of nitric oxide]." Biochemistry (Moscow, Russia). Vol. 61, no. 10. (January 1996.): 1731-42.

Khlestkin V, Mazhukin D, Tikhonov A, Bagryanskaja I, Gatilov Yu, Utepbergenov D, Khramtsov V V, Volodarsky LB. "Unexpected transfromation of 1,2-bis(N-methoxy-N-nitrosoamino)cycloalkanes: First synthesis of 4,5-dihydro-1,2,3-triazole 2-oxides." Tetrahedron Lett. Vol. 37, (January 1996.): 5997-6000.

Zamaraev, K, I; Romannikov, V, N; Salganik, R, I; Wlassoff, W, A; Khramtsov, V V. "Modelling of the prebiotic synthesis of oligopeptides: silicate catalysts help to overcome the critical stage.." Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life. Vol. 27, no. 4. (January 1997.): 325-37.

Khramtsov, V, V; Yelinova, V, I; Yelinova, V, I; Reznikov, V, A; Zimmer, G. "Quantitative determination and reversible modification of thiols using imidazolidine biradical disulfide label.." Journal of biochemical and biophysical methods. Vol. 35, no. 2. (January 1997.): 115-28.

Balakirev, M.Yu.; Khramtsov, V.V; Zimmer, G.. "Modulation of the mitochondrial permeability transition by nitric oxide.." European journal of biochemistry. Vol. 246, no. 3. (January 1997.): 710-718.

Haseloff, R, F; Kirilyuk, I, A; Dikalov, S, I; Khramtsov, V, V; Utepbergenov, D, I; Blasig, I, E; Grigor'ev, I A. "Synthesis and spin trapping applications of 2,2-dimethyl-d6-4-methyl-2H-imidazole-1-oxide-1-15N.." Free radical research. Vol. 26, no. 2. (January 1997.): 159-68.

Zwicker K, Dikalov S, Matuschka S, Mainka L, Hofmann M, Khramtsov V V, Zimmer G. "Oxygen Radical Generation and Enzymatic Properties of Mitochondria in Hypoxia/Reoxygenation." Arzn-Forschung/Drug Research. Vol. 48(1), (January 1998.): 629-636.

Paschenko SV, Dzuba SA, Khramtsov V V, Tsvetkov Yu D. "The Use of pH-Sensitive Nitroxide Spin Probe to Study Diffusion of Acids." Ber. Bumsenges. Phys. Chem. Vol. 102, (January 1998.): 224-228.

Kirilyuk IA, Utepbergenov DI, Mazhukin DG, Fechner K, Mertsch K, Khramtsov V V, Blasig IE and Haseloff RF. "Thiol-Induced Nitric Oxide Release from 3-Halogeno-3,4-dihydrodiazete 1,2-Dioxides." J. Med. Chem. Vol. 41(7), (January 1998.): 1027-1033.

Glazachev Yu I, Khramtsov V V, Berezina TA, Volodarsky LB. "Imidazoline biradical spin labels with EPR spectral sensitivity to the local concentration of protons." Applied Magn. Reson.. Vol. 15, (January 1998.): 407-415.

Khramtsov, V V; Berliner, L, J; Clanton, T L. "NMR spin trapping: detection of free radical reactions using a phosphorus-containing nitrone spin trap.." Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine. Vol. 42, no. 2. (January 1999.): 228-34.

Yelinova, V, I; Khramtsov, V, V; Markel, A L. "Manifestation of oxidative stress in the pathogenesis of arterial hypertension in ISIAH rats.." Biochemical and biophysical research communications. Vol. 263, no. 2. (January 1999.): 450-3.

Slepneva, I, A; Glupov, V, V; Sergeeva, S, V; Khramtsov, V, V. "EPR detection of reactive oxygen species in hemolymph of Galleria mellonella and Dendrolimus superans sibiricus (Lepidoptera) larvae.." Biochemical and biophysical research communications. Vol. 264, no. 1. (January 1999.): 212-5.

Hassanain, H, H; Sharma, Y, K; Moldovan, L; Khramtsov V V; Berliner, L, J; Duvick, J, P; Goldschmidt-Clermont, P J. "Plant rac proteins induce superoxide production in mammalian cells.." Biochemical and biophysical research communications. Vol. 272, no. 3. (January 2000.): 783-8.

Khramtsov, V, V; Grigor'ev, I, A; Foster, M, A; Lurie, D, J; Nicholson, I. "Biological applications of spin pH probes.." Cellular and molecular biology. Vol. 46, no. 8. (January 2000.): 1361-74.

Khramtsov, V, V; Reznikov, V, A; Berliner, L, J; Litkin, A, K; Grigor'ev, I, A; Clanton, T L. "NMR spin trapping: detection of free radical reactions with a new fluorinated DMPO analog.." Free radical biology & medicine. Vol. 30, no. 10. (January 2001.): 1099-107.

Markel', A, L; Elinova, V, I; Khramtsov V V. "[Oxidative stress in pathogenesis of arterial hypertension in ISIAH rats]." Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova / Rossiiskaia akademiia nauk. Vol. 87, no. 5. (January 2001.): 594-9.

Berliner, L.J., Khramtsov, V. V., Fujii, H., Clanton, T.L.. "Unique In Vivo Applications of Spin Traps." Free Radic. Biol. Med. Vol. 30, no. 5. (January 2001.): 489-499.

Sergeeva, S, V; Slepneva, I, A; Khramtsov, V, V. "Effect of selenolipoic acid on peroxynitrite-dependent inactivation of NADPH-cytochrome P450 reductase.." Free radical research. Vol. 35, no. 5. (January 2001.): 491-7.

Glazachev Yu I, Grigor'ev IA, Reijerse EJ, Khramtsov V V. "EPR Studies of 15N-and 2H-Substituted pH-Sensitive Spin Probes of Imidazoline and Imidazolidine Types." Applied Magn. Reson.. Vol. 20, (January 2001.): 489-505.

Berliner, LJ; Khramtsov, VV; Clanton TL; Fuji, H.. "NMR and MRI Spin Trapping: Using NMR to learn about free radical reactions." Current Topics in Biophysics. Vol. 26 (1), (January 2002.): 89-95.

Khramtsov, VV, Bobko AA, Popov SA, Rezinkov VA, Bagryanskaya EG, Clanton TL. "New series of fluorine-containing probes for NO detection using EPR and NMR spin trapping." Free Radical Biological Medicine. Vol. 33(Suppl 2), (January 2002.): S377-.

Khramtsov VV, Grigor'ev IA, Kirilyuk IA, Ilangovan G, Kuppusamy P. "In vivo EPR measurement of tissue acidosis during myocardial ischemia using pH-sensitive nitroxides." Free Radical Biological Medicine. Vol. 33(Suppl 2), (January 2002.): S423-S424.

Bhatt, Nitin, Y; Kelley, Todd, W; Khramtsov, Valery, V; Wang, Yijie; Lam, Gregory, K; Clanton, Thomas, L; Marsh, Clay B. "Macrophage-colony-stimulating factor-induced activation of extracellular-regulated kinase involves phosphatidylinositol 3-kinase and reactive oxygen species in human monocytes.." Journal of immunology. Vol. 169, no. 11. (January 2002.): 6427-34.

Potapenko, Dmitrii, I; Clanton, Thomas, L; Bagryanskaya, Elena, G; Gritsan, Nina, P; Reznikov, Vladimir, A; Khramtsov, Valery V. "Nonradical mechanism of (bi)sulfite reaction with DEPMPO: cautionary note for SO3*- radical spin trapping.." Free radical biology & medicine. Vol. 34, no. 2. (January 2003.): 196-206.

Potapenko DI, Bagryanskaya EG, Reznikov VV, Clanton TL, Khramtsov VV. "NMR and EPR studies of the reaction of nucleophilic addition of (bi) sulfite to the nitrone spin trap DMPO." Magn. Reson. Chem.. Vol. 41, (January 2003.): 603-608.

Foster, Margaret, A; Grigor'ev, Igor, A; Lurie, David, J; Khramtsov, Valery V; McCallum, Stephen; Panagiotelis, Ioannis; Hutchison, James M, S; Koptioug, Andrei; Nicholson, Ian. "In vivo detection of a pH-sensitive nitroxide in the rat stomach by low-field ESR-based techniques.." Magnetic resonance in medicine. Vol. 49, no. 3. (January 2003.): 558-67.

Kirilyuk IA, Shevelev TG, Morozov DA, Khromovskih EL, Skuridin NG, Khramtsov VV, Grigor'ev IA. "Grignard Reagent Addition to 5-Alkylamino-4H-Imidazole 3-Oxides: Synthesis of New pH-Sensitive Spin Probes." Synthesis. Vol. N 10, (January 2003.): 1-8.

Slepneva, Irina, A; Komarov, Denis, A; Glupov, Viktor, V; Serebrov, Valery, V; Khramtsov, Valery V. "Influence of fungal infection on the DOPA-semiquinone and DOPA-quinone production in haemolymph of Galleriamellonella larvae.." Biochemical and biophysical research communications. Vol. 300, no. 1. (January 2003.): 188-91.

Reznikov VA, Skuridin NG, Khromavskikh EL, Khramtsov VV. "A new series of lipophilis pH-sensitive spin probes." Russian Chemical Bulletin. Vol. 52(9), (January 2003.): 2052-2056.

Elinova, V, I; Bobko, A, A; Mazhukin, D, G; Markel', A, L; Khramtsov, VV. "New donors and acceptors of nitric oxide as a potential therapeutical agents." Bioorganicheskaia khimiia. Vol. 29, no. 4. (January 2003.): 434-41.

Lozinskaia, Ia, L; Slepneva, I, A; Khramtsov, V, V; Glupov, V V. "[Change of the antioxidant status and free radicals production in hemolymph of Galleria mellonella larvae in microsporidiosis.]." Zhurnal evoliutsionnoi biokhimii i fiziologii. Vol. 40, no. 2. (January 2004.): 99-103.

Bobko, Andrey, A; Bagryanskaya, Elena, G; Reznikov, Vladimir, A; Kolosova, Nataljya, G; Clanton, Thomas, L; Khramtsov, Valery V. "Redox-sensitive mechanism of no scavenging by nitronyl nitroxides.." Free radical biology & medicine. Vol. 36, no. 2. (January 2004.): 248-58.

Potapenko DI, Bagryanskaya EG, Tsentalovich Yu P, Reznikov VV, Clanton TL, Khramtsov V V. "Reversible reactions of thiols and thiyl radicals with nitrone spin traps." J. Phys. Chem. B.. Vol. 108, (January 2004.): 9315-9324.

Kirilyuk, Igor, A; Bobko, Andrey, A; Grigor'ev, Igor, A; Khramtsov, Valery V. "Synthesis of the tetraethyl substituted pH-sensitive nitroxides of imidazole series with enhanced stability towards reduction.." Organic & biomolecular chemistry. Vol. 2, no. 7. (January 2004.): 1025-30.

Khramtsov, Valery V; Grigor'ev, Igor, A; Foster, Margaret, A; Lurie, David J. "In vitro and in vivo measurement of pH and thiols by EPR-based techniques.." Antioxidants & redox signaling. Vol. 6, no. 3. (January 2004.): 667-76.

Khramtsov VV, Grigor'ev IA, Foster MA, Lurie DJ, Zweier JL, Kuppusamy P. "Spin pH and SH probes: enhancing functionality of EPR-based techniques." Spectroscopy. Vol. 18, (January 2004.): 213-225.

Komarov, Denis, A; Slepneva, Irina, A; Glupov, Viktor, V; Khramtsov, Valery V. "Superoxide and hydrogen peroxide formation during enzymatic oxidation of DOPA by phenoloxidase.." Free radical research. Vol. 39, no. 8. (January 2005.): 853-8.

Komarov, D.A., Slepneva, I.A., Glupov, V.V., and Khramtsov, V.V.. "Detection of free radicals formation in haemolymph of insects by EPR spectroscopy." Appl. Magn. Reson.. Vol. 28, (January 2005.): 411-419.

Kirilyuk, Igor, A; Bobko, Andrey, A; Khramtsov, Valery, V; Grigor'ev, Igor A. "Nitroxides with two pK values--useful spin probes for pH monitoring within a broad range.." Organic & biomolecular chemistry. Vol. 3, no. 7. (January 2005.): 1269-74.

Potapenko, D.I., Bagryanskaya, E.G., Grigoriev, I.A., Maksimov, A.M., Reznikov, V.A., Platonov, V.E., Clanton, T.L., and Khramtsov, V.V.. "Quantitative determination of SH-groups using 19F NMR spectroscopy and disulphide of 2,3,5,6-tetrafluoro-4-mercapto-benzoic acid." Magn. Reson. Chem.. Vol. 43, no. 11. (January 2005.): 902-909.

Voinov, M.A., Polienko, J.F., Schanding, T., Bobko, A.A., Khramtsov V V, Gatilov, Y.V., Rybalova, T.V., Smirnov, A.I., Grigor’ev, I.A.. "Synthesis, structure, and X-band (9.5 GHz) EPR characterization of the new series of pH-sensitive spin probes – N,N-Disubstituted 4-Amino-2,2,5,5-tetramethyl-3-imidazoline 1-Oxyls." J.Org.Chem.. Vol. 70, (January 2005.): 9702-9711.

Khramtsov, V. V.. "Biological Imaging and Spectroscopy of pH." Current Organic Chemistry. Vol. 9, (January 2005.): 909-923.

Khlestkin VK, Butakov VV, Grigor'ev IA, Bobko AA, Khramtsov VV. "Molecular design of pH-sensitive spin probes in the 2,3,4,6,7,8-hexahydroimidazo[1,5-a]pyrimidine series with different lipophilic/hydrophilic properties." Synthesis. Vol. 20, (January 2005.): 3649-3653.

Bobko, A.A., Sergeeva, S.V., Bagryanskaya, E.G., Markel, A.L., Khramtsov, V.V., Reznikov, V.A., Kolosova, N.G.. "19 F NMR measurements of NO production in hypertensive ISIAH and OXYS rats." Bioch. Biophys. Res. Commun.. Vol. 330, (January 2005.): 367-370.

Potapenko, D.I., Foster, M.A., Kirilyuk, I.A., Lurie, D.J., Hutchitson, J.M.S., Grigoriev, I.A., Bagryanskaya, E.G., and Khramtsov, V. V.. "Real-time monitoring of drug-induced changes in the stomach acidity of living rats using improved pH-sensitive nitroxides and low-field EPR techniques." J. Magn. Reson.. Vol. 182, (January 2006.): 1-11.

Komarov, D, A; Slepneva, I, A; Dubovskii, I, M; Grizanova, E, V; Khramtsov, V, V; Glupov, V V. "Generation of superoxide radical and hydrogen peroxide in insect hemolymph in the course of immune response.." Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections / translated from Russian. Vol. 411, (January 2006.): 482-5.

Glazachev, Yu, I; Khramtsov V V. "Fluorescence recovery under decaying photobleaching irradiation: concept and experiment.." Journal of fluorescence. Vol. 16, no. 6. (January 2006.): 773-81.

Amelkin, M.A., Glazachev Y.I., Khramtsov V V.. "Study of the localization of a fluorescence probe on the membrane surface using angle-resolved fluorescence depolarization method." Biologicheskie Membrany (Russian). Vol. 23, no. 3. (January 2006.): 244-250.

Bobko, Andrey, A; Kirilyuk, Igor, A; Grigor'ev, Igor, A; Zweier, Jay, L; Khramtsov, Valery, V. "Reversible reduction of nitroxides to hydroxylamines: roles for ascorbate and glutathione.." Free radical biology & medicine. Vol. 42, no. 3. (January 2007.): 404-12.

Wang, Yijie; Zeigler, Mandy, M; Lam, Gregory, K; Hunter, Melissa, G; Eubank, Tim, D; Khramtsov, Valery, V; Tridandapani, Susheela; Sen, Chandan, K; Marsh, Clay B. "The role of the NADPH oxidase complex, p38 MAPK, and Akt in regulating human monocyte/macrophage survival.." American journal of respiratory cell and molecular biology. Vol. 36, no. 1. (January 2007.): 68-77.

Swartz, H.M., Khan, N., and Khramtsov, V. V... "Use of Electron Paramagnetic Resonance Spectroscopy to Evaluate the Redox State In Vivo." Antioxid. Redox Signal. Vol. 9, no. 10. (January 2007.): 1757-1771.

Dhimitruka I, Velayutham M, Bobko AA, Khramtsov V V, Villamena FA, Hadad CM, and Zweier, JL.. "Large-scale synthesis of a persistent trityl radical for use in biomedical EPR applications and imaging." Bioorg. Medicinal Chem. Lett.. Vol. 17, (January 2007.): 6801-6805.

Samouilov, A., Zweier, J.L., Woldman, Ya., Khramtsov, V. V.. "Magnetic Resonance Study of the Transmembrane Nitrite Diffusion." Nitric Oxide. Vol. 16, (January 2007.): 362-370.

Bobko AA, Dhimitruka I, Zweier JL, Khramtsov V V.. "Trityl radicals as persistent dual function pH and oxygen probes for in vivo electron paramagnetic resonance spectroscopy and imaging: concept and experiment." J. Am. Chem. Soc.. Vol. 129,, no. 23. (January 2007.): 7240-7241.

Polovyanenko DN, Plyusnin VF, Reznikov VA, Khramtsov V V, Bagryanskaya EG.. "Mechanistic Studies of the Reactions of Nitrone Spin Trap PBN with Glutathiyl Radical." J. Phys. Chem. B.. Vol. 112, (January 2008.): 4841-4847.

Roshchupkina GI, Bobko AA, Bratasz A, Reznikov VA, Kuppusamy P and Khramtsov V V.. "In vivo EPR measurement of glutathione in tumor-bearing mice using improved disulfide biradical probe." Free Radic. Biol. Medicine. Vol. 45, (January 2008.): 312-320.

Bratasz A, Selvendiran K, Wasowicz T, Bobko A, Khramtsov V V, Ignarro LJ and Kuppusamy P. "NCX-4040, a nitric oxide-releasing aspirin, sensitizes drug-resistant human ovarian xenograft tumors to cisplatin by depletion of cellular thiols." Journal of Translational Medicine. Vol. 6, no. 9. (January 2008.): -.

Fomenko EV, Bobko AA, Salanov AN, Kirilyuk IA, Grigor'ev IA, Khramtsov V V, Anshits AG.. "Perforated cenosphere-supported pH-sensitive spin probes." Russian Chem. Bulletin. Vol. 57, (January 2008.): 493-498.

Dhimitruka I, Bobko AA, Hadad C, Zweier J, Khramtsov V V.. "Synthesis and Characterization of Amino Derivatives of Persistent Trityl Radicals as Dual Function pH and Oxygen Paramagnetic Probes." J. Am. Chem. Soc.. Vol. 130, no. 32. (January 2008.): 10780-10787.

Bobko AA, Dhimitruka I, Eubank TD, Marsh CB, Zweier JL and Khramtsov V V.. "Trityl-based EPR probe with enhanced sensitivity to oxygen." Free Radic. Biol. Medicine. Vol. 47, (January 2009.): 654-658.

Woldman YY, Sun J, Zweier JL, and Khramtsov V V.. "Direct Chemiluminescent Detection of Nitric Oxide in Aqueous Solutions Using Natural Nitric Oxide Target, Soluble Guanylyl Cyclase." Free Radic.Biol. Medicine. Vol. 47, (January 2009.): 1339-1345.

Woldman Y.Y., Semenov S.V., Bobko A.A., Kirilyuk I.A., Polienko J.F., Voinov M.A., Bagryanskaya E.G. and Khramtsov, V. V.. "Design of liposome-based pH sensitive nanoSPIN probes: nano sized particles with incorporated nitroxides." The Analyst. Vol. 134, (January 2009.): 904-910.

Komarov, D.A., Ryazanova, A.D., Slepneva, I.A., Khramtsov, V. V., Dubovskiy, I.M., Glupov, V.V.. "Pathogen-targeted hydroxyl radical generation during melanization in insect hemolymph: EPR study of probable cytotoxicity mechanism." Appl. Magn. Reson.. Vol. 35, (January 2009.): 495-501.

Dhimitruka, I., Grigorieva, O., Zweier, J.L., Khramtsov, V. V.. "Synthesis, structure and EPR characterization of deuterated derivatives of Finland trityl radical." Bioorganic & Medicinal Chemistry Letters. Vol. 20, (January 2010.): 3946-3949.

Bobko AA, Kirilyuk IA, Gritsan NP. Polovyanenko DN, Grigor’ev IA, Khramtsov VV, Bagryanskaya EG.. "EPR and quantum chemical studies of the pH-sensitive imidazoline and imidazolidine nitroxides with bulky substituents." Appl Magn Reson. Vol. 39, (January 2010.): 437-451.

Khramtsov V V, Caia GL, Shet K, Kesselring E, Petryakov S, Zweier JL and Samouilov A.. "Variable Field Proton-electron double-resonance imaging: application to pH-mapping of aqueous samples." J. Magn. Reson.. Vol. 202, (January 2010.): 267-273.

Efimova OV, Sun Z, Petryakov S, Kesselring E, Caia GL, Johnson D, Zweier JL, Khramtsov VV, Samouilov A. "Variable Radio Frequency Proton-Electron Double-Resonance Imaging: application to pH mapping of aqueous samples." J. Magn. Reson. Vol. 209, no. 2. (January 2011.): 227-232.

Khramtsov, V.V.; Clanton, T.L.. "NMR Spin Trapping: Insight into the Hidden Life of Free Radical Adducts." Appl. Magn. Reson. Vol. 41, (January 2011.): 305-323.

Bobko, AA, Efimova, OV, Voinov, MA, and Khramtsov, VV.. "Unique oxidation of imidazolidine nitroxides by potassium ferricyanide: strategy for designing paramagnetic probes with enhanced sensitivity to oxidative stress." Free Radic. Research.. Vol. 46, (September 2012.): 1115-1122.

Komarov, D.A.; Ilirian, D.; Kirilyuk, I.A.; Trofimov, D.G.; Grigor'ev, I.A.; Zweier, J.L.; Khramtsov, V.V.. "Electron paramagnetic resonance monitoring of ischemia-induced myocardial oxygen depletion and acidosis in isolated rat hearts using soluble paramagnetic probes." Magn. Reson. Med.. Vol. 68, (August 2012.): 649-655.

Bobko, AA, Dhimitruka, I, Komarov, DA, and Khramtsov, VV.. "Dual function pH and oxygen phosphonated trityl probe." Analytical Chemistry. Vol. 84, (July 2012.): 6054-6060.

Koda, S; Goodwin, J; Khramtsov, VV; Fujii, H; Hirata, H.. "Electron paramagnetic resonance-based ph mapping using spectral-spatial imaging of sequentially scanned spectra." Analytical chemistry. Vol. 84, (March 2012.): 3833-3837.

Ahmad, R. Potter, L.C., Khramtsov, V.V.. "Spectral modeling for accelerated pH spectroscopy using EPR." J. Magn. Reson.. Vol. 218, (March 2012.): 86-92.

Bobko, AA, Ivanov, A, Khramtsov VV.. "Discriminative EPR detection of NO and HNO by encapsulated nitronyl nitroxides." Free Radical Research. Vol. 47, no. 2. (February 2013.): 74-81.

Dhimitruka, I, Bobko AA, Eubank, TD, Komarov, DA, and Khramtsov, VV.. "Phosphonated trityl probes for concurrent in vivo tissue oxygen and pH monitoring using electron paramagnetic resonance-based techniques." J. Am. Chem. Soc.. Vol. 135, (March 2013.): 5904-5910.

Warshaviak, DT, Khramtsov VV, Cascio D, Altenbach C, and Hubbell WL. "Structure and dynamics of an imidazoline nitroxide side chain with strongly hindered internal motion in proteins." Journal of Magnetic Resonance. Vol. 232, (July 2013.): 53-61.



"Imidazoline and imidazolidine nitroxides as spin probes for ESR monitoring of local chemmical environment in vivo." Presented at International workshop on Techniques and Bio-Medical Applications of In Vivo EPR and PEDRI, Aberdeen. (September 1999)

"Imidazoline Nitroxides in Spin Labeling: Combining Functional Chemical Reactivity and Stability of Nitroxyl Fragment." Presented at 6th Internaltional Symposium on Spin Tapping, Marseille. (August 2000)

"New Approached in Spin Labeling and Spin Trapping." Presented at International Summer School on Biophysics, Rovinj, HR|HRV. (September 2000)

"New NO donors and acceptors as potential therapeutic agents." Presented at AvHumboldt Russian-German Conference on Biomedicine, Moscow. (November 2001)

"In vivo and in vitro EPR studies using imidazoline nitroxide spin probes." Presented at International workshop on In Vivo EPR and Related Studies, Hannover, NH. (September 2001)

"Enhanced opportunities in design of spin labeling experiment using imidazoline nitroxides." Presented at 3rd International Conference on Nitroxide Radicals, Kaiserslautern, DE|DEU. (September 2001)

"Magnetic Resonance Spin Trapping: from EPR towards NMR." Presented at 35th Annual Meeting of EPR groups of the Royal Society of Chemistry, Aberdeen. (April 2002)

"NMR Spin trapping of free radicals: limitations, advantages and future directions." Presented at 7th International Symposium on Spin Trapping, Chapel Hill, NC, US|USA. (July 2002)

"Spin pH and SH probes: enhancing functionality of EPR-based techniques." Presented at 2nd International Conference on Biomedical Spectroscopy, London, GB|GBR. (July 2003)

"Mechanism of antagonistic action of nitronyl nitroxides against nitric oxide in reducing environment: in vitro and in vivo studies." Presented at 5 th Meeting of the European Federation of EPR Groups, Lisbon, PT|PRT. (September 2003)

"31P and 19F NMR spin trapping of short-lived intermediates." Presented at 48th Annual Meeting of Biophysical Society, Baltimore, MD, US|USA. (February 2004)

"New insight into the mechanism of the redox equilibrium of the nitroxide-hydroxylamine couple: the role for ascorbate and glutathione." Presented at 11th Annual Meeting of Society for Free Radical Biology & Medicine, St. Thomas, VI, US|USA. (November 2004)

"Mechanistic studies of the reaction of the nitronyl nitroxides with NO and superoxide." Presented at 4th Intern. Conf. On Peroxynitrite and Reactive Nitrogen Species in Biology and Medicine, Konstanz, DE|DEU. (July 2004)

"Enhanced functionality of EPR-based techniques using pH and SH probes." Presented at International Workshop on in vivo EPR, Hanover, NH, US|USA. (September 2004)

"Antioxidant mechanisms of the nitroxides and nitrones." Presented at 2nd International Meeting of the Society for Free Radical Research – Asia & The Third International Symposium on Natural Antioxidants- Molecular Mechanisms and Health Effects, Shanghai, CN|CHN. (June 2005)

"New developments in functionality of electron paramagnetic resonance probes for free radical biology and medicine." Presented at 11th in vivo EPR spectroscopy and imaging & 8th International EPR spin trapping meeting, Columbus, OH, US|USA. (September 2005)

"Biological imaging and spectroscopy of pH: important applications for pH-sensitive nitroxides." Presented at 4th International Conference on nitroxide radicals, Novosibirsk. (September 2005)

"Thiol-specific nitroxides for site directed spin labeling and EPR measurements of thiol content." Presented at XXIInd International Conference on Magnetic Resonance in Biological Systems, Goettingen, DE|DEU. (August 2006)

"New insight into the antioxidant mechanisms of PBN spin trap." Presented at 13th Meeting of the Society for Free Radical Research International, Davos, CH|CHE. (August 2006)

"Application of pH-Sensitive Probes for Measuring Ischemia-induced Myocardial Acidosis: an in vivo L-band EPR Spectroscopy Study." Presented at 5th Asia-Pacific EPR/ESR Symposium, Novosibirsk. (August 2006)

"Trityl radicals as persistent dual function pH and oxygen probes." Presented at 14th Annual Meeting of Society for Free Radical Biology & Medicine, Washington, DC, US|USA. (November 2007)

"Functional EPR spectroscopy and imaging using nitroxides and trityl radicals." Presented at 2nd International Conference on Frontiers in Vascular Medicine, Melbourne, AU|AUS. (October 2007)

"The development of new functional nitroxide probes for noninvasive EPR spectroscopy and imaging." Presented at International Meeting EPR 2007, Chicago, IL, US|USA. (May 2007)

"Progress in functional EPR probes: trityl and nitroxyl radicals." Presented at Biomedical Redox Navigation (EPR 2008), Fukuoka, JP|JPN. (September 2008)

"Biomedical EPR spectroscopy and imaging of nitroxides." Presented at 5th International Conference on Nitroxide Radicals, Ancona, IT|ITA. (September 2008)

"The reaction of nitrone spin trap PBN with glutathiyl radical: probable antioxidant mechanism." Presented at Society of Free Radical Research European meeting, Berlin, DE|DEU. (July 2008)

"EPR measurement of glutathione redox state." Presented at 6th Asia Pacific EPR Symposium, Cairns, AU|AUS. (July 2008)

"In vivo EPR measurement of glutathione in tumor-bearing mice using disulfide nitroxide biradical." Presented at International Conference on Magnetic Resonance in Biological Systems, San Diego, CA, US|USA. (August 2008)

"Functional mapping using variable field proton electron double resonance imaging." Presented at International Euromar Magnetic Resonance Conference, Goeteborg, SE|SWE. (July 2009)

"In vivo multifunctional EPR spectroscopy using nitroxyl and trityl paramagnetic probes." Presented at 6th International conference on Reactive oxygen and nitrogen species, antioxidants and human health, Smolensk. (September 2009)

"In vivo extracellular pH monitoring in tumor tissues of PyMT mice: effect of GM-CSF treatment." Presented at 16th Annual Meeting of the Society for Free Radical Biology & Medicine, San Francisco, CA, US|USA. (November 2009)

"Functional Proton Electron Double Resonance Imaging: concept and experiment." Presented at 17th Meeting of International Society for Magnetic Resonance in Medicine, Honolulu, HI, US|USA. (April 2009)

"Multifunctional in vivo EPR using nitroxyl and trityl radicals." Presented at EPR2010: A Joint Conference of the 14th In Vivo EPR Spectroscopy & Imaging and the 11th International EPR Spin Trapping/Spin Labeling, San Juan, PR, US|USA. (May 2010)

"Multifunctional EPR spectroscopy using nitroxyl probes and nanoSPINs: nanoSized Particles with Incorporated Nitroxides." Presented at The International Chemical Congress of Pacific Basin Societies, Honolulu, HI. (December 2010)


Professional Activities

1990 - present Free radical Biology and Medicine, J. of American Chemical Society, Biophysical Journal, Biochemistry, Biochim.Biophys.Acta, American Journal of Physiology, Circulation, Biochem. Pharmacol., J.Phys.Chem., and others..
1998 - present Russian Foundation of Fundamental Sciences (moscow, Russia); U.S. Civilian Research & Development Foundation (Arlington, Virginia); National Institutes of Health (USA).
1998 Conference organization. German-Russian symposium on EPR application in biomedicine.
2005 International EPR 2005 conference. Columbus, OH.
2005 4th International Conference on Nitroxide Radicals.
2006 5th Asia-Pacific EPR/ESR Symposium.
2009 International Conference on Reactive Oxygen Species, Nitric Oxide, Antioxidants, and Human Health.
2011 International Conference on Reactive Oxygen Species, Nitric Oxide, Antioxidants, and Human Health.