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Bradford S. McGwire, MD, PhD


Staff photo

410 W 10th Ave
Columbus, OH 43210

Phone: (614) 293-0431

Email: mcgwire.2@osu.edu

 

Current OSU Appointments

Assistant Professor, Infectious Diseases

Physician, FGP-Infectious Diseases

Assistant Professor, SBS-Microbial Infectn/Immunity

 

Partnership Narrative

1) Developed informal partnership with Helix Biomedix which is an antimicrobial peptide company in Bothell, WA. They have donated several antimicrobial peptides to the lab for anti-parasitic testing.



2) I have an informal partnership with Pfizer who donated the anti-fungal compound voriconazole (VFEND) for anti-leishmanial testing. This has been published in Parasitology Research in 2013 (see publication list).

Published Works

The main thrust of my publications concern studies on the cellular and molecular biology of the pathogenic trypanosomatid protozoa (Leishmania, Trypanosoma cruzi and Trypanosoma brucei). In the last several years they have have dealt with the interactions of these parasites with antimicrobial peptides (innate immune system effectors). The results have implications for the role of AMPs in the natural control of parasite infection in the infected host (insect and mammalian) as well as for the development of AMPs as chemotherapeutic agents. We have identified two key AMP resistance mechanisms of parasites (gp63 metelloprotease expression in Leishmania and Cyp19 expression in T. cruzi). I have published some assorted reviews and chapters on aspects of parasite biology and chemotherapeutics. I have also published a few clinical case reports.

Research Funding

During my time at OSU I have been awarded 3 grants; 2 from the American Heart Association (Beginning Grant-in-Aid and Scientist Development Grant) and an internal award from OSU (Davis Bremer). I have written numerous NIH (R01 and R21), NSF, AHA grants as well as a variety of brants to other agencies such as the Burroughs Wellcome Fund. I have been awarded a 2013-2014 Fulbright Scholar Award to develop collaborations in Ecuador on Chagas disease. Most recently I was invited to submit a fill proposal to the NSF base on my recent work on Chagas. This is based on the "high quality" score on a pre-proposal (22% of 288 proposals were invited). I also received funding through the OSU-FAPESP(Brazil) Collaboration to fund work with my collaborator in Brazil from 2014-2015 on T. cruzi signalling. I was recently awarded a 2 year (2014-2016) Grant-in-Aid from the AHA for my lab at OSU.

Academic Advising

Periodically I advise medical students, residents and fellows in training about career options in medicine. I draw upon my personal experience in clinical medicine and in basic and translational science in order to discuss opportunities they meay wish to consider.

Biographical

I am currently an Assistant Professor (clinical) of Medicine in the Division of Infectious Diseases at The Ohio State University. I earned my PhD degree (mentor: Prof. K.-P. Chang) in 1995 in the area of Microbiology/Immunology (with a focus in molecular parasitology) from the University of Health Sciences/Chicago Medical School in Illinois. I earned my MD degree from the same institution in 1997. I completed a clinical investigator-track pathway combined internal medicine residency and infectious disease fellowship from the University of Illinois-Chicago (UIC)/University of Chicago in 2003. I did the research portion of this fellowship in the area of molecular parasitology at Northwestern University (mentor: Prof. David Engman) where I was a post-doctoral fellow and then a visiting scholar from 2003-2004. During the same time was a clinical instructor of medicine working as an infectious disease attending on the faculty at UIC. In 2004, I joined the faculty in Infectious Disease Division at OSU as a tenure-track Assistant Professor and switched to the clinical-track in April 2013 on the advice of P and T committee and re-oriented my career into the clinical scholar pathway.



Since 2004 I have been increasingly engaged in service as an attending in the area of general clinical infectious diseases in all of the OSU hospitals (Main, Ross, James and East) providing consultative services and well as serving as an attending on the ID inpatient ward (until its closure in 2013). I also regularly serve infectious disease patients in the outpatient clinic seeing referrals as well as hospital follow-ups. Since 2008, I have also been involved in managing ID issues in the prison population in the OSU telemedicine service. In addition to diagnosis and management of general infectious disease I am the general “go to” person for issues regarding parasitic-related infectious disease, both among my colleagues as well as seeing outside referrals. My other service-oriented activities include serving on the Lab Utilization and Test Committee for the hospital, the ID Division Finance Committee and the Academic Advisory Committee for Fellow Research for the ID Division.



In 2004, I also started my basic research laboratory working in the area of the molecular parasitology of the parasitic protozoans Leishmania and trypanosomes (T. cruzi and T. brucei), and have been a member of the Center for Microbial Interface Biology (CMIB) since starting at OSU. My work has been funded by several research grants from the American Heart Association, most recently receiving a 2 year “Grant-in-Aid” award starting in July 2014 through 2016. My lab has been successful, publishing numerous papers on our work in impactful journals such as Journal of Biological Chemistry, Cellular Microbiology, Molecular Microbiology and Infection and Immunity. I have written several reviews and book chapters on the molecular biology of Trypanosoma cruzi and Leishmania and clinical diagnosis and treatment of leishmaniasis and American trypanosomiasis. I have developed collaborations among my colleagues at OSU as well as nationally and internationally. My work has been recognized internationally and I have presented in national and international forums. I have served as grant reviewer on several national and international committees, notably the Medical Research Council (UK), the Portuguese Foundation for Science and Technology and the German Research Foundation as well as the American Heart Association and the US Dept. of Defense. I serve as ad hoc reviewer on numerous scientific journals. I have recently started international collaborations related to the my research work and have received a 2013-2014 Fulbright Scholar Award from the US Department of State to collaborate on field work on Chagas Disease (caused by T. cruzi) on Ecuador. I have also received an OSU-FAPESP pilot grant for collaboration with a collaborator in Brazil on T. cruzi signalling. Overall, my research activities are currently funded from 3 sources.



I have been engaged in teaching in both the clinical and basic science arenas. Since 2004 I have taught the medical parasitology section of the host defense block in the medical student curriculum. I have also translated this into the new LSI curriculum where I teach the parasitology and anti-parasitic chemotherapy sections. Multiple times per year, on each clinical rotation, I teach medical students, medical residents and infectious disease fellows concepts of clinical diagnostic and therapeutics management of infectious disease topics encountered on the wards. This is both as didactic settings and while seeing patients. Semi-annually I also give medical resident lectures on “Unusual Infections”. In the last couple of years I have also served as an evaluator of medical students performing Objective Structured Clinical Examinations (OSCEs) on standardized patients. I have routinely served to interview incoming ID fellow candidates and have served as an evaluator for potential MSTP students. I give annual lectures in the graduate level Microbial Pathogenesis (IGBP course 7724), Infectious Disease in the Developing World (PUBH-EPI 5412) and lectures in the ID Signature Series: Translational Science-Infection & Host Pathogen Science (IBGP 8800.02). This year I became the course director of this section of this seminar series.



Overall, I have been actively engaged in the service, research and teaching missions of the university. My goal moving forward is to continue to remain active in each of these areas and to further expand my level of activity. With respect to expansion in the area of clinical service, I have become involved in hepatitis C treatment, which is a new area for the division. In regards to research, I have recently expanded my collaborative activities internationally to South America which will undoubtedly lay the groundwork for future collaborative studies and grant funding and may serve as a platform for additional educational opportunities for our ID fellows. In the area of teaching, I have taken as course director for a translational science seminar series which paves the way for having a positive impact on science education.

Continuing Education, Extension, and Other Courses Taught

I have given Grand Rounds to the Dept of Medicine at OSU in 2008 entitled "Antimicrobial Peptides in Medicine and Disease". I give annual journal club presentations to the ID Division on topics related to ID. I generally present translational science topics which are intended to bridge the gap from basic and clinical science. This is important especially for the ID fellow trainees in the audience. I also give semi-annual talks to the medcial residents on "Unusual Infections". I also give periodic talks on research in my lab to the ID division. In addition I give didactic lectures to the residents and medical students on the ID consultation service on various ID topics which have included HIV, Antibiotics and TB/pneumonia.

Advising Narrative

Tulasi Gude, MD was in my lab for a short period of time during her undergraduate work. She is co-author on one paper on voriconizole activity against Leishmania.

Master/Graduate Advising

I have served on the PhD thesis committees of two graduate students: Hannah Cummings, PhD (2011, advisor Abhay Satoskar) and Kimberly Newkirk (2007, advisor Michaal Lairmore).

Academic Advising

2012 - present Ken Woods, The Ohio State University.
2012 - present Bonnie Prokesch, The Ohio State University.
2013 - present Patricia Salvador, The Ohio State University.
2013 - present Muhammed Ali The Ohio State University.
2013 - present Vidhya Chandrasekaran, The Ohio State University.
2004 - 2005 Jamal Horani, The Ohio State University. Graduated 2005.
2004 - 2006 Jeremy Young, The Ohio State University. Graduated 2006.
2004 - 2006 Niveditha Reddy, The Ohio State University. Graduated 2006.
2007 Kimberly Newkirk, The Ohio State University.
2006 - 2008 Raed Khairy, The Ohio State University. Graduated 2008.
2006 - 2008 Nikkiya Fraser, The Ohio State University. Graduated 2008.
2007 - 2009 Eric Jones, The Ohio State University. Graduated 2009.
2007 - 2009 Jose Bazan, The Ohio State University. Graduated 2009.
2007 - 2009 Mark Lustberg, The Ohio State University. Graduated 2009.
2008 - 2009 Tulasi Gude, The Ohio State University.
2008 - 2010 Rachel Demita, The Ohio State University. Graduated 2010.
2008 - 2010 Gabriel Galang, The Ohio State University. Graduated 2010.
2008 - 2010 Legesse Mekonnen, The Ohio State University. Graduated 2010.
2009 - 2010 Alissa Ackelson. The Ohio State University. Graduated 2010.
2009 - 2010 Madhuri Sopirala, The Ohio State University. Graduated 2010.
2009 - 2011 Doug Haas The Ohio State University. Graduated 2011.
2009 - 2011 Richard Bakker, The Ohio State University. Graduated 2011.
2009 - 2011 Christina Liscynesky, The Ohio State University. Graduated 2011.
2011 Hannah Cummings, The Ohio State University.
2005 - 2012 Manjusha Kulkarni, The Ohio State University.
2010 - 2012 Satish Sarvepalli, The Ohio State University. Graduated 2012.
2010 - 2012 Julian Goodman, The Ohio State University. Graduated 2012.
2010 - 2012 Foad Abandeh, The Ohio State University. Graduated 2012.
2011 - 2013 Robert Leininger, The Ohio State University. Graduated 2013.
2011 - 2013 Amber Vasquez, The Ohio State University. Graduated 2013.
2012 - 2013 Sid Hublikar, The Ohio State University.
 

Certifications

2006 - present Board Certification: American Board of Internal Medicine
2007 - present Board Certification: American Board of Internal Medicine
1997 - 2004 MD: State Medical Board Illinois
2004 - 2017 MD: State Medical Board of Ohio
 

Chapters in Books

Bradford S. McGwire, David M. Engman. "American Trypanosomiasis." In Medical Parasitology. Edited by Abhay R. Satoskar, Gary L. Simon, Peter J. Hotez, Moriya Tsuji. -. Austin, TX: Landes Bioscience, January 2010.

McGwire, BS. "Treatment modalities for cutaneous and visceral leishmaniasis." In Pathogenesis of Leishmaniasis: New Developments in Research. Edited by Satoskar, AS, Durvasula, R.. -. New York, US|USA: Springer Science+Business Media, January 2014.

 

Clinical Services

07/01/2004 Attending Physician (Outpatient Infectious Disease Clinic (McCampbell Hall and previously Cramblett Hall))
07/01/2004 Attending Physician (Inpatient ID service-Main Hospital)

Degrees

1995 Ph.D., University of Health Sciences/Chicago Medical School

1997 M.D., University of Health Sciences/Chicago Medical School

 

Editorial Activities

2004 - present Parasitology
2004 - present Biochimie
2004 - present Research in Microbiology
2004 - present Biochemical Journal
2004 - present Antimicrobial Agents and Chemotherapy
2004 - present Annals of Clinical Microbiology and Antimicrobials
2004 - present American Journal of Tropical Medicine & Hygiene
2004 - present Microbes and Infection
2004 - present Journal of Infectious Diseases
2004 - present Journal of Clinical Microbiology
2004 - present Journal of Bacteriology
2004 - present Clinical Infectious Diseases
2004 - present Biology of the Cell
2004 - present Infection and Immunity
2004 - present Experimental Parasitology
2004 - present ACTA Tropica
2004 - present Eukaryotic Cell
2005 - present European Journal of Protistology
2005 - present Protist
2005 - present Molecular Microbiology
2006 - present Molecular and Biochemical Parasitology
2007 - present PLOS-Neglected Tropical Diseases
2011 - present Folia Parasitologia
2011 - present Open Journal of Medical Microbiology
2011 - present PLOS-One
 

Honors

2013 - 2014 Fulbright Scholar Award. Fulbright Commission of the US.
2013 - 2015 OSU-Brazil (FAPESP) Award. Ohio State University.
 

Journal Articles

McGwire BS. "Review of Nucleic acid hybridization: A new tool for the diagnosis of infectious disease.." STUDENT DOctor. Vol. 10, no. 1. (January 1988.): 20-.

Stipkovits L, Belak S, McGwire BS, Ballagi-Pordany A, Santha M. "Rapid identification of Mycoplasma gallisepticum using a simple method of nucleic acid hybridization.." Mol. Cell. Probes. Vol. 2, no. 4. (January 1988.): 339-44.

Morein B, Belak S, Soos T, Rusvai M, McGwire BS, Bognar K. "Iscom of viral envelope proteins protects against Aujeszky's disease.." Vet. Microbiol.. Vol. 20, no. 2. (January 1989.): 143-54.

Sears AE, McGwire BS, Roizman B. "Infection of polarized MDCK cells with herpes simplex virus 1: two asymmetrically distributed cell receptors interact with different viral proteins.." Proc. Nat. Acad. Sci. USA. Vol. 88, no. 12. (January 1991.): 5087-91.

Mensa-Wilmot K, LeBowitz JH, Chang KP, Al-Qahtani A, McGwire BS, Tucker S, Morris JC. "A glycosylphosphatidylinositol (GPI)-negative phenotype produced in Leishmania major by GPI phospholipase C from Trypanosoma brucei: topography of two GPI pathways.." J. Cell Biol.. Vol. 124, no. 6. (January 1994.): 935-47.

Mensa-Wilmot K, LeBowitz JH, Chang KP, al-Qahtani A, McGwire BS, Tucker S, Morris JC.. "GPI phospholipase C from Trypanosoma brucei causes a GPI-negative phenotype in Leishmania major: I. Implications for GPI-negative mammalian cells; II. Compartmentalization of two GPI biosynthetic pathways.." Brazil. J. of Med. Biol. Res.. Vol. 27, no. 2. (January 1994.): 177-84.

McGwire B, Chang KP. "Genetic rescue of surface metalloproteinase (gp63)-deficiency in Leishmania amazonensis variants increases their infection of macrophages at the early phase.." Mol. Biochem. Parasit.. Vol. 66, no. 2. (January 1994.): 345-7.

Singh S, McGwire BS, Chang KP. "KINETOPLAST AND NUCLEAR-DNA POLYMORPHISM OF VISCERAL LEISHMANIA ISOLATES FROM NORTHEASTERN INDIA." JOURNAL OF PROTOZOOLOGY RESEARCH. Vol. 5, no. 1. (January 1995.): 27-32.

Brittingham A, Morrison CJ, McMaster WR, McGwire BS, Chang KP, Mosser DM. "Role of the Leishmania surface protease gp63 in complement fixation, cell adhesion, and resistance to complement-mediated lysis.." J. Immunol.. Vol. 155, no. 6. (January 1995.): 3102-11.

Chakrabarty R, Mukherjee S, Lu HG, McGwire BS, Chang KP, Basu MK. "Kinetics of entry of virulent and avirulent strains of Leishmania donovani into macrophages: a possible role of virulence molecules (gp63 and LPG).." J. Parasitol.. Vol. 82, no. 4. (January 1996.): 632-5.

McGwire BS, Chang KP. "Posttranslational regulation of a Leishmania HEXXH metalloprotease (gp63). The effects of site-specific mutagenesis of catalytic, zinc binding, N-glycosylation, and glycosyl phosphatidylinositol addition sites on N-terminal end cleavage,." J. Biol. Chem.. Vol. 271, no. 14. (January 1996.): 7903-9.

Mensa-Wilmot K, Garg N, McGwire BS, Lu, HG, Zhong L, Armah DA, LeBowitz JH, Chang KP. "Roles of free GPIs in amastigotes of Leishmania.." Mol. Biochem. Parasit.. Vol. 99, no. 1. (January 1999.): 103-16.

Brittingham A, Chen G, McGwire BS, Chang KP, Mosser DM. "Interaction of Leishmania gp63 with cellular receptors for fibronectin.." Infect. Immun.. Vol. 67, no. 9. (January 1999.): 4477-84.

Chang KP, McGwire BS.. "Molecular determinants and regulation of Leishmania virulence.." Kinetoplastid Biol Dis.. Vol. 1, no. 1. (January 2002.): 1-.

McGwire BS, O'Connell WA, Chang KP, Engman DM. "Extracellular release of the glycosylphosphatidylinositol (GPI)-linked Leishmania surface metalloprotease, gp63, is independent of GPI phospholipolysis: implications for parasite virulence.." J. Biol. Chem.. Vol. 277, no. 11. (January 2002.): 8802-9.

McGwire BS, Chang KP, Engman DM. "Migration through the extracellular matrix by the parasitic protozoan Leishmania is enhanced by surface metalloprotease gp63.." Infect. Immun.. Vol. 71, no. 2. (January 2003.): 1008-10.

McGwire BS, Olson CL, Tack BF, Engman DM. "Killing of African trypanosomes by antimicrobial peptides." JOURNAL OF INFECTIOUS DISEASES. Vol. 188, no. 1. (July 2003.): 146-152.

Chang KP, Reed SG, McGwire BS, Soong L. "Leishmania model for microbial virulence: the relevance of parasite multiplication and pathoantigenicity.." ACTA Tropica. Vol. 85, no. 3. (January 2003.): 375-390.

Young, JD, McGwire, BS. "Infliximab and reactivation of cerebral toxoplasmosis.." The New England journal of medicine. Vol. 353, no. 14. (January 2005.): 1530-1; discussion 1530.

Kulkarni MM, McMaster WR, Kamysz E, Kamysz W, Engman DM, McGwire BS. "The major surface-metalloprotease of the parasitic protozoan, Leishmania, protects against antimicrobial peptide-induced apoptotic killing.." Mol. Microbiol.. Vol. 62, no. 5. (January 2006.): 1484-97.

Kulkarni MM, Jones EA, McMaster WR, McGwire BS. "Fibronectin binding and proteolytic degradation by Leishmania and effects on macrophage activation." Infect Immun. Vol. 76, no. 4. (April 2008.): 1738-1747.

Kulkarni MM, Olson CL, Engman DM, McGwire BS. "Trypanosoma cruzi GP63 Proteins Undergo Stage-Specific Differential Posttranslational Modification and Are Important for Host Cell Infection." INFECTION AND IMMUNITY. Vol. 77, no. 5. (May 2009.): 2193-2200.

Kulkarni MM, McMaster WR, Kamysz W, McGwire BS. "Antimicrobial peptide-induced apoptotic death of leishmania results from calcium-de pend ent, caspase-independent mitochondrial toxicity.." J. Biol. Chem.. Vol. 284, no. 23. (January 2009.): 15496-15504.

McGwire BS, Kulkarni MM. "Interactions of antimicrobial peptides with Leishmania and trypanosomes and their functional role in host parasitism." EXPERIMENTAL PARASITOLOGY. Vol. 126, no. 3. (November 2010.): 397-405.

Maric D, McGwire BS, Buchanan KT, Olson CL, Emmer BT, Epting CL, Engman DM. "Molecular Determinants of Ciliary Membrane Localization of Trypanosoma cruzi Flagellar Calcium-binding Protein." JOURNAL OF BIOLOGICAL CHEMISTRY. Vol. 286, no. 38. (September 2011.): 33109-33117.

Kulkarni MM, Barbi J, McMaster WR, Gallo RL, Satoskar AR, McGwire BS. "Mammalian antimicrobial peptide influences control of cutaneous Leishmania infection.." Cell. Microbiol.. Vol. 13, no. 6. (January 2011.): 913-923.

Marr AK, McGwire BS, McMaster WR. "Modes of action of Leishmanicidal antimicrobial peptides." FUTURE MICROBIOLOGY. Vol. 7, no. 9. (September 2012.): 1047-1059.

McGwire B, Marr B, Zhou X-P, Ayers L. "Iliopsoas coccidioidomycotic abscess with associated intra-abdominal extension in an immunocompetent patient.." BMJ Case Reports. Vol. 2012, (January 2012.): 1-3.

Kulkarni MM, Karafova A, Kamysz W, Schenkman S, Pelle R, McGwire BS. "Secreted trypanosome cyclophilin inactivates lytic insect defense peptides and induces parasite calcineurin activation and infectivity.." J Biol Chem.. Vol. 288, no. 12. (February 2013.): 8772-84.

Kulkarni MM, Reddy N, Gude T, McGwire BS.. "Voriconazole suppresses the growth of Leishmania species in vitro.." Parasitol Res.. Vol. 112, (February 2013.): 2095–2099-.

McGwire BS. "Acute abdominal pain and diarrhoea in healthy adult." British Medical Journal Case Reports. Vol. doi: 10.1136/bcr-2013-009441, (June 2013.): doi: 10.1136/bcr-2013.

McGwire BS and Satoskar AR. "Leishmaniasis: clinical syndromes and treatment." Quarterly J Med.. Vol. 107, no. 1. (January 2014.): 7-14.

Kulkarni MM, Karafova A, Kamysz W, McGwire BS. "Design of protease-resistant pexiganan enhances antileishmanial activity." Parasitology Research. Vol. 113, (April 2014.): 1971–1976-.

Walker D, Gupta G, McGwire BS, Drew M, Satoskar AR. "Molecular mechanisms of invasion by parasitic protozoa." Cellular and Molecular Life Sciences. Vol. 71, no. 7. (April 2014.): 1245-63.

 

Softwares

July 2013 Council for the International Exchange of Scholars."Fulbright Scholar Program" CIES
July 2013 OSU-Office of International Affairs."14 FROM OHIO STATE EARN FULBRIGHT SCHOLAR GRANTS FOR 2013-2014" OSU-Office of International Affairs
September 2013 OSU-FAPESP."OSU-Brazil grants"

Inventions and Patents

Bradford S. McGwire. "Novel antibacterial protein from Trypanosoma cruzi." OSU. Filed September 2010.

Bradford S. McGwire. "Antimicrobial activity secreted from entomopathogenic bacteria." OSU. Filed September 2010.

 

Presentations

"Host origin of replication in the genome of HSV-1.." Presented at VIIIth International Congress of Virology, Berlin, DE|DEU. (January 1990)

"Studies on herpes simplex virus latency." Presented at Howard Hughes Medical Institute - National Institutes of Health, Bethesda, MD, US|USA. (January 1990)

"Extrachromosomal genetics in trypanosomatid biology." Presented at VIIIth Meeting of the Brazilian Society of Protozoology, Rio De Janerio, BR|BRA. (January 1992)

"Functional analysis of Leishmania metalloproteinase by genetic modifications.." Presented at University of Health Science - Research Poster Session, N. Chicago, IL, US|USA. (January 1993)

"Leishmania in medicine and research." Presented at ACCA biology seminar series, Lemont, IL, US|USA. (January 1993)

"Functional analysis of Leishmania metalloproteinase (gp63) by site-specific mutagenesis.." Presented at University of Health Science - Dept of Microbiology, N. Chicago, IL, US|USA. (January 1993)

"Extrachromosomal expression of Leishmania gp63 genes and their site specific mutants.." Presented at International Congress of Parasitology, Izmir, TR|TUR. (January 1994)

"Structure, function and evolution of the ecto-metalloprotease (gp63) of Leishmania. Molecular analysis of wildtype genes and their site-specific mutants.." Presented at University of Health Science - Research Poster Session, N. Chicago, IL, US|USA. (January 1995)

"Posttranscriptional regulation of leishmanial ecto-metalloprotease (gp63) expression and activity in the parasitism of macrophages.." Presented at Calandra Research Forum - Department of Pathology, Chicago, IL, US|USA. (January 1995)

"Molecular genetic analysis of leishmanial ecto-metalloprotease (gp63): Posttranscriptional regulation of enzyme expression and activity in the paraitism of macrophages.." Presented at University of Health Science - Dept of Microbiology, N. Chicago, IL, US|USA. (January 1995)

"Functional analysis of an HEXXH metalloprotease (gp63) by site-sp[ecific mutagenesis.." Presented at University of Health Science - Research Poster Session, N. Chicago, IL, US|USA. (January 1996)

"Inactivation of gp63 proteolytic activity by site-specific mutation diminishes the intramacrophage survival of Leishmania amazonensis." Presented at 3rd Internet Conference on Salvarian Trypanosomes and Trypanosomatids, Chicago, IL, US|USA. (January 2000)

"Posttranscriptional regulation of Leishmania ectometalloprotease (gp63) gene expression via concerted interaction of putative elements in the 5' - 3' flanking regions.." Presented at Molecular Parasitology Meeting, Woods Hole, MA, US|USA. (January 2000)

"Leishmania gp63 is released extracellularly via two different mechanisms." Presented at Molecular Parasitology Meeting, Woods Hole, MA, US|USA. (January 2000)

"Expression of Trypanosoma cruzi FCaBP in Leishmania reveals a conserved mechanism for flagellar protein lozalization." Presented at 3rd Internet Conference on Salvarian Trypanosomes and Trypanosomatids, Chicago, IL, US|USA. (January 2000)

"Indentification of surface localized gp63 homologues in all life stages of Trypanosoma cruzi." Presented at Molecular Parasitology Meeting, Woods Hole, MA, US|USA. (January 2001)

"Release of gp63 and diminished in vitro early phase infection of a GPI - lacking mutant from Leishmania mexicana." Presented at WorldLeish2 Meeting, Crete, GR|GRC. (January 2001)

"Cell Surface autoproteolytic and secretory release of leishmanial metalloprotease (gp 63)." Presented at WorldLeish2 Meeting, Crete, GR|GRC. (January 2001)

"Dissection and conservation of the mechanism of flagellar localization of Trypanosoma cruzi FCaBP." Presented at Molecular Parasitology Meeting, Woods Hole, MA, US|USA. (January 2001)

"Indentification of surface localized T. cruzi gp63 homologues in all life stages and is involved in host parasitism." Presented at Molecular Parasitology Meeting, Woods Hole, MA, US|USA. (January 2003)

"Killing Of African trypanosomes by antimicrobial peptides.." Presented at 11th International Infectious Diseases, Cancun, MX|MEX. (January 2004)

"Indentification of surface localized T. cruzi gp63 homologues in all life stages and is involved in host parasitism." Presented at 11th International Infectious Diseases, Cancun, MX|MEX. (January 2004)

"Membrane active peptieds as potential anti-parasite therapeutics." Presented at 45th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC), New Orleans, LA, US|USA. (January 2005)

"Antileishmanial/trypanosomal activities of antimicrobial peptides.." Presented at Gordon Conference, Antimicrobial Peptides, Ventura, CA, US|USA. (January 2005)

"Antimicrobial peptide resistance by the parasitic protozoan Leishmania: a novel function for the surface metalloprotease and its implications for the design of surface active chemotherapeutics." Presented at 4th Annual OSUMC Research Day, Columbus, OH, US|USA. (January 2005)

"Rapid and extensive metallo and cysteine proteolytic degradation of the extracellular matrix proten, fibroectin by insect and intracellular forms of Leishmania contributes to enhanced macrophage infection.." Presented at Midwest Microbial Pathogenesis Conference, Cincinnati, OH, US|USA. (January 2006)

"Antimicrobial peptide (AMP)-Leishmania interactions:importance of the parasite surface-protease in evasion of AMP-induced apoptotic cell death and a novel role for AMPs in parasite pathogenesis." Presented at Midwest Microbial Pathogenesis Conference, Cincinnati, OH, US|USA. (January 2006)

"Parasite surface-metalloprotease protects against antimicrobial peptide induced apoptotic killing of Leishmania." Presented at Dept. of Microbiology and Immunology, Chicago, IL, US|USA. (January 2006)

"Differential induction of apoptotic and non-apoptotic death of Leishmania by distinct classes of antimicrobial peptides." Presented at CMIB Retreat at OSUMC, Columbus, OH, US|USA. (January 2007)

"Differential induction of apoptotic and non-apoptotic death of Leishmania by distinct classes of antimicrobial peptides." Presented at Kinetoplastid Molecular Cell Biology Meeting, Woods Hole, OH, US|USA. (January 2007)

"Fibronectin binding and metallo- and cysteine proteolytic degradation by Leishmania contributes to parasite infectivity." Presented at CMIB Retreat at OSUMC, Columbus. (January 2007)

"Fibronectin binding and metallo- and cysteine proteolytic degradation by Leishmania contributes to parasite infectivity." Presented at Kinetoplastid Molecular Cell Biology Meeting, Woods Hole, MA, US|USA. (January 2007)

"Antimicrobial peptide “sensing” by the pathogenic protozoan Trypanosoma cruzi leads to differentiation of insect-stage parasites to infectious forms." Presented at Midwest Microbial Pathogenesis Conference, Chicago, IL, US|USA. (January 2007)

"Antimicrobial peptide “sensing” by the pathogenic protozoan Trypanosoma cruzi leads to differentiation of insect-stage parasites to infectious forms." Presented at CMIB Retreat at OSUMC, Columbus, OH, US|USA. (January 2007)

"Characterization of a protein from Trypanosoma cruzi as a potential novel anti-microbial compound." Presented at CMIB Retreat at OSUMC, Columbus, OH, US|USA. (January 2008)

"Antimicrobial Peptides in Medicine and Disease." Presented at Internal Medicine Grand Rounds, Columbus, OH, US|USA. (January 2008)

"Characterization of a protein from Trypanosoma cruzi as a potential novel anti-microbial compound." Presented at 7th Annual OSUMC Research Day, Columbus, OH, US|USA. (January 2008)

"Differential induction of apoptotic death of Leishmania by antimicrobial peptides results from calcium-dependent mitochondrial toxicity." Presented at World Congress on Leishmaniasis, Lucknow, IN|IND. (January 2009)

"The murine cathelicidin-related antimicrobial peptide, CRAMP, influences control of cutaneous leishmaniasis; 4th World congress on Leishmaniasis." Presented at World Congress on Leishmaniasis, Lucknow, IN|IND. (January 2009)

"The murine cathelicidin-related antimicrobial peptide, CRAMP, influences control of cutaneous leishmaniasis." Presented at Midwest Microbial Pathogenesis Conference, West Lafayette, IN, US|USA. (January 2009)

"Antimicrobial peptide “sensing” by the pathogenic protozoan Trypanosoma cruzi leads to differentiation of insect-stage parasites to infectious form." Presented at Midwest Microbial Pathogenesis Conference, St. Louis, MO, US|USA. (January 2010)

"Novel anti-microbial proteins against MRSA and Francisella novicida." Presented at GLRCE Regional Meeting, Chicago, IL, US|USA. (January 2010)

"Secreted trypanosomal cyclophilin mediates host antimicrobial peptide evasion promoting parasite survival and infectivity." Presented at CMIB Retreat at OSUMC, Columbus, OH, US|USA. (January 2011)

"The murine cathelicidin-related antimicrobial peptide, cramp, influences control of cutaneous leishmaniasis." Presented at 4th Annual T2C Conference on Inflammation Tissue Injury and wound repair, Columbus, OH, US|USA. (January 2011)

"Secreted trypanosome cyclophilin mediates calcineurin-based sensing and evasion of insect defense peptide." Presented at 2nd Annual Midwest Neglected Infectious Disease Meeting, South Bend, IN, US|USA. (August 2012)

"Secreted trypanosome cyclophilin inactivates lytic insect defense peptides and induces calcineurin activation and infectivity." Presented at Cyclophilins and other Foldases: Cell Signaling Catalysts and Drug Targets, Halle, DE|DEU. (September 2013)

"Case Presentation: IDSO 2014." Presented at Infectious Disease Society of Ohio, Columbus, OH, US|USA. (April 2014)

"Case Presentation; IDSO 2014." Presented at Infectious Disease Society of Ohio 2014, Columbus, OH, US|USA. (April 2014)

 

Professional Activities

2004 - present Infectious Disease Clinical Consultation. OSU Hospitals.
2004 - present Ad Hoc Reveiwer. Multiple scientific journals.
2004 - present Inpatient Infectious Diseases Service. OSU Main Hospital.
2008 - present Grant Review. American Heart Association.
2008 - present German Research Foundation.
2009 - present Grant Reviewer. Medical Research Council (British).
2009 - present Grant Reveiwer. Portuguese Foundation for Science and Technology.
2011 - present Parasitiology Journal/Research Club.
2013 - present Grant Reviewer. Department of Defense.
 

Unpublished Works

Kulkarni, M, Varikuti, S, Satoskar, AS, McGwire, BS. Stat-1 is critical to the control of Trypanosoma cruzi infection. April 2014.